Apr 29, 2019 · Mechanistically, EPR interacts with chromatin and regulates Cdkn1a gene expression by affecting both its transcription and mRNA decay through its association with SMAD3 and the mRNA decay-promoting factor KHSRP, respectively.

We propose that EPR downregulation upon 6 h of TGF-β treatment causes SMAD3 dismissal from Cdkn1a promoter that results in a return of Cdkn1a transcription to basal levels and, in parallel, enables KHSRP to destabilize the Cdkn1a transcript .

In support of the critical role of TGF-β signaling in epilepsy, several studies have discovered the downstream target gene CDKN1A as a central hub in epileptogenesis. A transcriptional analysis of rat piriform cortex following sarin-induced seizures identified several significant canonical pathways and de novo networks of genes most ...

Relative expression of HMGB1, TGF-β1, BIRC3, ADAM17, CDKN1A, and FTO in controls and MI patients’ PBMCs. Relative mRNA expression is presented as 2 −∆Ct value for each sample. The cDNAs from the PBMCs if controls (N = 24) and patients with MI (N = 95) were used to quantify gene expression.

Oct 2, 2024 · TGF-β1 and BIRC3 mRNA were significantly lower in patients compared to controls (p = 0.02 and p = 0.05, respectively). Conclusions: Our results suggest that HMGB1 is involved in adverse LV remodeling six-months-post-MI, even on the mRNA level. Further research and validation are needed.

Feb 26, 2015 · To test whether p21 mediates TGF-β-induced drug resistance, we conducted in vivo KDs by introducing Cdkn1a shRNAs into our LV TGF-β reporter constructs (Figure 7B). In Cdkn1a KD tumors, p21 expression was abrogated in TGF-β reporter + cells.

Jul 1, 2009 · We have shown that the activation of the Smad and nonSmad signaling pathways can be modulated by the expression level of TGFBR2 and that the activation state of the nonSmad signaling pathway principally determines whether TGF-β can induce CDKN1A/p21 mediated apoptosis in an epithelial cell line.

May 5, 2010 · CDKN1A encoding p21 Cip1 has been identified as a Gfi-1 target gene and shown to contain Gfi-1 binding sites in the upstream promoter region. We show here that Gfi-1 represses CDKN1A in a manner that is independent of its DNA binding activity.

Apr 29, 2019 · Mechanistically, EPR interacts with chromatin and regulates Cdkn1a gene expression by affecting both its transcription and mRNA decay through its association with SMAD3 and the mRNA...

Nov 27, 2006 · The cyclin-dependent kinase inhibitor 1A (CDKN1A), also known as p21 (WAF1/CIP1) modulates cell cycle, apoptosis, senescence and differentiation via specific protein–protein interactions with the cyclins, cyclin-dependent kinase (Cdk), and many others.