May 1, 2022 · In this study, we show that RAB7 is required for lysosomal degradation of the EGF·EGFR complex. Upon dissecting the endocytic pathway of RAB7-deficient cells, we find that cargo can proceed through EEA1 (early endosome antigen 1)-positive early endosomes and into CD63-positive LE/MVB.

Raw264.7 macrophages transfected with two plasmids encoding either EGFP-fused CD63 or RILP and a dominant-negative form of Rab7, Rab7T22N, were allowed to phagocytose latex-beads for 2 hr and were then examined by CLSM for localization of …

RAB7, LAMP-1 and CD63 are localized onto late endosomes/MVBs and they are downregulated in chemoresistant cells. RAB7 downregulation causes chemoresistance by increasing release of CD9- and CD81-positive exosomes.

In this study, we observe that Rab7 is markedly recruited to LDs in live hepatocellular carcinoma cells when placed under nutrient deprivation. Most importantly, Rab7 is highly activated under these conditions and is required to promote direct physical interactions between MVBs/lysosomes and …

Apr 29, 2020 · In this study, we improved the stability and brightness of superecliptic pHluorin-CD63 by incorporating a single amino acid mutation, M153R, previously identified to stabilize ratiometric...

Mar 1, 2013 · Rab7 designates the maturation of endosomes and also autophagosomes, directing the trafficking of cargos along microtubules, and finally, participating in the fusion step with lysosomes. Rab7 is an effective multifunctional regulator of autophagy and endocytosis.

Here, it has been demonstrated that a lysosomal glycoprotein, CD63, is recruited to the majority of M.tb phagosomes, while RILP shows limited localization. This is consistent with the author's findings that CD63, but not RILP, is recruited to the phagosomes in macrophages expressing the dominant negative form of Rab7.

Mar 29, 2019 · Mechanistic studies reveal that LE transport effector RILP prefers ubiquitylation-deficient Rab7, while retromer-mediated LE recycling benefits from an intact cycle of Rab7 ubiquitylation.

anti-CD63 antibody. Immunoblotting analysis revealed that CD63 is recruited to live M.tb phagosomes as well as to heat-inactivated M. smegmatis phagosomes. These results suggest that M.tb phagosomes fuse with CD63-positive lysosomal vesicles. RILP interacts with the active form of Rab7 and me-diates the fusion of endosomes with lysosomes (14, 15).

Nov 3, 2020 · We find that active Rab7 interacts with the NPC1 cholesterol transporter and licenses lysosomal cholesterol export. This process is abolished in C18orf8 -, Ccz1 - and Mon1A/B -deficient cells and...