Anti-CD3 scFv dimers expressed on the cell sur-face induced CD25 (IL-2 receptor a-chain) expression and proliferation of splenocytes. CT26 tumor cells engineered to express surface scFv dimers...

To bridge the gap between cytotoxic T lymphocytes and tumor cells, our objective in this study was to describe the construction and the cell surface-anchored expression of a fusion protein, anti-CD3 scFv-B7.1, derived from inserting a fusion gene encoding anti-CD3 scFv and the extra-cellular domain of B7.1 fused by the splicing by overlap ...

Feb 17, 2000 · Anti-CD3 scFv dimers expressed on the cell surface induced CD25 (IL-2 receptor α-chain) expression and proliferation of splenocytes. CT26 tumor cells engineered to express surface scFv dimers...

Weaker binding to CD3 was observed for BiTEs with anti-CD3 scFv in V L-linker-V H configuration, especially when it is located on the C-terminal side of the molecule (i.e., VL CD19-VH CD19-VL CD3-VH CD3), whereas anti-CD19 scFv showed a strong binding to target cells regardless of the location or domain order . Similar effects of molecular ...

Feb 1, 2004 · Single-chain Fv (scFv) monomers, dimers and trimers were generated by changing the linker length between the V H and V L domains. The linkers used were the natural extensions of the V H into the C H 1 domain. The dimeric molecules …

Mar 30, 2023 · To enhance the therapeutic index of T-cell engagers (TCEs), we engineered masked, precision-activated TCEs (XPAT proteins), targeting a tumor antigen (human epidermal growth factor receptor 2...

Here, we describe a bispecific single-chain Fv (scFv) immunofusion or BIf to target CD123(+) leukemia, that contains an anti-CD123 scFv fused at the N-terminus of human IgG1 hinge-C(H)2-C(H)3, and an anti-CD3 scFv fused at C-terminus.

May 31, 2023 · We modified T cells with a fusion protein of anti-GPC3 single-chain fragment variable (scFv) linked to CD3ε, which incorporates into the native T cell receptor/CD3 complex forming chimeric anti-GPC3 scFv-CD3ε engineered T cells (CT0180).

May 1, 2019 · In this study, we manufactured anti-B7-H4/CD3 bispecific antibodies (BsAb) based on the Fab and scFv structure. We found that the B7-H4/CD3 BsAb had potent cytotoxic activity against B7-H4 + breast cancer cell lines in vitro, and in vivo using a …

Jun 3, 2021 · scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas, but phage display is a popular way to generate scFvs. For phage display, a pooled library of scFvs is displayed on the coats of bacteriophages, a process which links genotype and phenotype.