Mar 3, 2022 · We verified that the anti-human CD3 Fabs in our panel, hCD3.1–hCD3.5 as well as SP34, bound potently to cyno and hCD3E/D, but not to mCD3E/D. Additionally, we used the murine surrogate anti-CD3...

Mar 17, 2025 · SP34 CD3 binding to CD3ε initiates structural and biochemical changes that drive T-cell signaling. This interaction stabilizes the TCR complex in a signaling-ready state, exposing critical tyrosine residues within ITAMs of CD3ε, CD3γ, CD3δ, and CD3ζ.

Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone.

Apr 20, 2022 · A comprehensive review of the literature shows that many early TCE drug developers relied on three primary mouse-derived anti-CD3 antibodies: OKT3, SP34, and UCHT1 (14 – 17).

Using insights from the crystal structure of anti-Hu/Cy CD3 antibody ADI-26906 in complex with CD3ε and antibody engineering using a yeast-based platform, we have derived high-affinity CD3 antibody variants with very low polyreactivity and significantly improved biophysical developability.

Mar 30, 2023 · The CD3-binding domain in HER2-XPAT protein is a proprietary variant of SP34 that was engineered for increased stability and reduced affinity for CD3.

Using a site-specific recombination strategy, anti-Her2 (2Rs15d) or anti-VEGFR2 (3VGR19) nanobodies were fused to anti-CD3 Fab (SP34) at different positions to generate various formats of Her2/CD3 and VEGFR2/CD3 bsAbs.

Jun 2, 2021 · Among these anti-CD3 antibodies, SP34 demonstrates subnanomolar binding affinity (0.17 nM) to CD3ε, and importantly, it shows cross-reactivity with cynomolgus monkey CD3, making in vivo toxicity studies in nonhuman primates feasible.

Activation of T cells with antibodies targeting human CD3 (clone SP34) induced CD4 and CD8 T cell proliferation, as well as production of IL-2 and IFN-gamma. The CD3 functionality was demonstrated in vitro by the ability of B cells to produce IgM upon activation of T cells, suggesting a proper cooperation between T and B cells.

Two antibodies, anti-human TCR gammadelta (B1.1), and anti-human CD3 (SP34) were found to costain in 13 species of nonhuman primates. This study has identified valuable new reagents for studying T cell populations in different animal species and for the first time characterized antibodies useful for studying gammadelta T cell populations in ...