CD177 is a neutrophil-specific receptor presenting the proteinase 3 (PR3) autoantigen on the neutrophil surface. CD177 expression is restricted to a neutrophil subset, resulting in CD177 pos /mPR3 high and CD177 neg /mPR3 low populations.

Feb 27, 2017 · Using flow cytometry and a PR3-specific FRET assay, we found that CD177 binding reduced the proteolytic activity of PR3 in vitro using purified proteins, in neutrophil degranulation supernatants...

In addition to β2 integrins and PR3, CD177 recognizes platelet endothelial cell adhesion molecule 1 (PECAM-1), an immunoglobulin-family molecule expressed on endothelial cells as well as platelets and certain leukocytes, including neutrophils themselves.

The mPR3 + /CD177 + phenotype as well as mRNA expression of PR3 and CD177 are increased in neutrophils from patients with AASV and SLE, while in PV patients only the mRNA expression of CD177 is increased. The increased levels of PR3 and CD177 are not related to plasma levels of G-CSF or GM-CSF.

Mar 18, 2021 · The reactivity of iso- and auto-antibodies against CD177 was studied using granulocytes, “native” CD177/PR3 complex, and recombinant CD177 or PR3. Results. All iso- and auto-antibodies were reactive with CD177/PR3 when …

Sep 3, 2021 · To date, the only confirmed (nonsubstrate) interaction partner for PR3 is CD177, a glycosylphosphatidylinositol-anchored protein with no clearly defined function. Because of its high-affinity complex with PR3, CD177 is responsible for the abundant mPR3 occurring in …

Mar 4, 2011 · The glycosylphosphatidylinositol (GPI)-anchored neutrophil-specific receptor NB1 (CD177) presents the autoantigen proteinase 3 (PR3) on the membrane of a neutrophil subset. PR3-ANCA-activated neutrophils participate in small-vessel vasculitis.

Using flow cytometry and a PR3-specific FRET assay, we found that CD177 binding reduced the proteolytic activity of PR3 in vitro using purified proteins, in neutrophil degranulation supernatants containing wtPR3 and directly on mPR3 high neutrophils and PR3-loaded HEK cells.

Aug 31, 2021 · CD177 is a neutrophil-specific receptor presenting proteinase 3 (PR3) autoantigen on the neutrophil surface. CD177 expression is restricted to a neutrophil subset resulting in CD177 pos /mPR3 high and CD177 neg /mPR3 low populations.

Proteinase 3 (PR3) is found in granules of all neutrophils but also on the plasma membrane of a subset of neutrophils (mPR3). CD177, another neutrophil protein, also displays a bimodal surface expression.