In healthy individuals, 8.6% ± 0.6 of the monocytes co-expressed CD56, with the majority of CD56+ monocytes being CD14 bright (7.9% ± 0.5), while only a minor population was CD14 dim (0.7% ± 0.1). We found a strong positive correlation between an individual’s age and the frequency of CD56+ monocytes.

Three main isoforms exist of CD56 (NCAM-120, NCAM-140, and NCAM-180), all generated by alternative splicing from one single gene, differing in their intracellular domain length (1). CD56 is often considered a marker of neural lineage commitment due to its discovery site (2).

To improve the monocyte purity, we devised a new gating scheme that takes advantage of the shared coexpression of cell surface markers on each subset. In addition to CD14 and CD16, CCR2, CD36, HLA-DR and CD11c are the most informative markers that discriminate among the three monocyte populations.

Feb 1, 2022 · The CD14 + CD56 + monocyte population (Fig. 1A, 1B) was significantly higher in moderate (4.71 ± 3.7; p < 0.01) and severe participants (5.58 ± 2.6; p < 0.005) compared with mild COVID-19 participants (2.07 ± 1.02) and uninfected participants (>1%).

Aug 15, 2022 · In the current study, we identified a circulating atypical monocyte subpopulation characterized by the expression of the transmembrane molecule CD56 (neural cell adhesion molecule) that displayed a consistent hyperinflammatory profile.

CD56 (low)CD33+ cells, primed with cytomegalovirus antigen, induced autologous T-lymphocyte proliferation comparably to CD56-, CD14+ peripheral blood monocytes (PBM). Conversely, CD56 (low) cells induced greater T-cell proliferation than CD56- PBM when lymphocyte responders were HLA mismatched.

Feb 15, 2024 · We analyzed cryopreserved PMBC samples using a 10-color flow cytometry panel to evaluate NK cell subpopulations in 31 individuals with sporadic PD and 27 HC participants. Here we identified...

CD14 is typically negative on monoblasts; however, some anti-CD14 clones recognize mature monocytic cells only while others recognize promonocytes and mature monocytes (Yang et al 2005). Understanding the performance characteristics of the anti-CD14 clone you use in your laboratory is critical.

Jul 26, 2012 · Monocytes and their subsets can be reliably identified using an antibody-fluorochrome “backbone” of HLA-DR, CD14, and CD16. CD16 pos monocytes continue to constitutively express HLA-DR even in conditions where HLA-DR is pathologically reduced on CD14 hi /CD16 neg monocytes.

Oct 1, 2013 · In healthy individuals, 8.6% ± 0.6 of the monocytes co-expressed CD56, with the majority of CD56+ monocytes being CD14bright (7.9% ± 0.5), while only a minor population was CD14dim (0.7% ± 0.1). We found a strong positive correlation between an individual’s age and the frequency of CD56+ monocytes.