Jan 30, 2023 · BrdU labeling can be performed in vitro for cell lines and primary cell cultures or in vivo for labeling cells within a living animal. During the BrdU assay, BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies.

BrdU (32.5 mM, 10 mg/mL): 5 x 1 mL sterile vials; store at less than or equal to -70°C. Avoid multiple freeze-thaws. Vial should be opened under sterile conditions.

With the availability of new highly specific anti-BrdU antibodies, the BrdU labeling technique can be combined with click chemistry detection for a simplified method of dual pulse labeling.

BrdU incorporation and detection in vivo protocol 022410 Method A. BrdU injections 1. Inject n mice with 1.6 mg BrdU in 200 µl i.p. (stock 20 mg/ml, 1:2.5 dilution = 8 mg/ml => 1.6 mg/200 µl). 2. 10-12 hrs later inject same mice with 1.2 mg BrdU in 200 µl i.p. (1:3.33 dilution of 20 mg/ml stock). B. BrdU detection – Day 1 3.

Step-by-step protocol for the use of BrdU (5-Bromo-2´-Deoxyuridine) to detect DNA synthesis using a fluorescence microscope.

Bromodeoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is ...

BrdU labeling can be performed on cell lines and primary cell cultures in vitro, and on cells in living animals in vivo. During the BrdU assay, BrdU is incorporated into replicated DNA and can be detected using anti-BrdU antibodies.

BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine normally used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro or in vivo. During the BrdU assay, BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies.

Labeling tumor cells with BrdU, either in vitro or in vivo, followed by detection with specific anti-BrdU monoclonal antibodies, is an accurate and comprehensive method for quantifying the degree of DNA synthesis.

Objectives: To investigate the optimal condition of bromodeoxyuridine (BrdU) labeling for bone marrow mesenchymal stem cells (BMSCs) in vitro and the feasibility of in vivo tracing of BrdU-labeling BMSCs. Methods: BMSCs were isolated from Wistar rats and were in …