Biuret (/ ˈbjurɛt / BYUR-ret) is a chemical compound with the chemical formula HN (CONH2)2. It is a white solid that is soluble in hot water. A variety of organic derivatives are known.

May 22, 2024 · This colorimetric chemical test used to detect the peptide bond using the Biuret reagent is called the Biuret test. It is also called Piotrowski’s reaction after the name of the Polish physiologist Gustaw Piotrowski who observed this phenomenon in 1857 and used it to detect proteins in samples.

In chemistry, the biuret test (IPA: / ˌ b aɪ j ə ˈ r ɛ t /, / ˈ b aɪ j ə ˌ r ɛ t / [1]), also known as Piotrowski's test, is a chemical test used for detecting the presence of at least two peptide bonds in a molecule.

Biuret is a member of the class of condensed ureas that is the compound formed by the condensation of two molecules of urea; the parent compound of the biuret group of compounds. Used as a non-protein nitrogen source in ruminant feed.

Jun 4, 2024 · The Biuret test is a chemical test used to determine the presence of peptide bonds in a sample. It is named after the French chemist Jean Baptiste Biuret, who first described the reaction in 1884. The test involves adding a copper (II) ion solution (such as …

Biuret is a colorimetric technique used for proteins and peptides, where copper salts in alkaline solution create a purple complex with substances containing two or more peptide bonds. This method is suitable for determining total protein concentration in a reaction system.

Feb 19, 2023 · The Biuret Test for Protein is based on the principle that copper ions in the Biuret reagent react with peptide bonds to form a violet-coloured complex. The Biuret reagent contains sodium hydroxide, copper sulphate, and potassium sodium tartrate.

The biuret test is a colorimetric test that helps detect specific proteins or peptide bonds in given analytes. It is followed by spectrophotometry for quantification. The test requires the use of a biuret reagent.

The biuret reaction is based on the complex formation of cupric ions with proteins. In this reaction, copper sulfate is added to a protein solution in strong alkaline solution. A purplish-violet color is produced, resulting from complex formation between the cupric ions and the peptide bond.

The Biuret Test is a fundamental biochemical method for detecting the presence of proteins in a sample. It works on the principle of peptide bond detection, where proteins react with copper ions in an alkaline solution to produce a violet colour.