Escherichia coli BL21 (DE3) is a commonly used protein production strain of the E. coli bacterium. This strain combines several features that allow for excessive expression of heterologous proteins. It is derived from the B lineage of E. coli. [1]

Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain. Ideal for routine T7 expression; Protease deficient B strain; No dry ice surcharge on competent cell shipments; Outgrowth medium included; Free of animal products

Jan 11, 2016 · BL21 (DE3) is suitable for expression from a T7 or T7-lac promoter or promoters recognized by the E.coli RNA polymerase: e.g. lac, tac, trc, ParaBAD, PrhaBAD and also the T5 promoter.

Thermo Scientific™ BL21(DE3) Competent Cells are suitable for the expression of nontoxic heterologous genes. The strain contains the DE3 lysogen that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase …

Oct 28, 2024 · The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase.

HI-Control BL21(DE3) Chemically Competent Cells Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor. Key features

Aug 12, 2021 · Escherichia coli BL21 (DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance...

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system. This product contains genetically modified organisms (GMO).