A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. [1][2][3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.

3 days ago · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.

What is a BAC library? BAC stands for Bacterial Artificial Chromosome. These are small, often circular, pieces of bacterial DNA that can be identified and copied within a bacterial cell. They can be used to store stretches of DNA from other organisms.

Jul 24, 2024 · Explore the structure, cloning process, and genomic applications of bacterial artificial chromosomes in this comprehensive guide. Bacterial Artificial Chromosomes (BACs) offer a powerful tool in genetic research and biotechnology.

Here, the present report describes the strategic considerations in creating and amplifying a genetically stable full-length infectious JEV cDNA as a bacterial artificial chromosome (BAC) using the following general experimental procedures: viral RNA isolation, cDNA synthesis, cDNA subcloning and modification, assembly of a full-length cDNA, cDNA...

A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a ...

In this chapter, we first discuss the general guidelines and cautions for preparing microinjection-quality DNA, and then describe in detail two protocols, one for gel purification of transgenic fragments from plasmid vectors and the other for isolating high-quality BAC DNA from bacteria.

Here we present a new way of performing BAC end sequencing on the CEQTM 8000 Genetic Analysis System from Beckman Coulter with high degrees of accuracy, automation, and throughput. The same system could be applied to perform end sequencing of other large DNA con-structs such as PAC, YAC, or cosmid, etc.

The BAC system is based on the well-characterized Escherichia coli F-factor, a low copy plasmid that exists in a supercoiled circular form in host cells. The structural features of the F-factor allow stable maintenance of individual human DNA clones as …

The introduction of bacterial artificial chromosome (BAC) libraries has made it easy for the scientific world to gain access to an unlimited amount of DNA from different species. These BAC libraries are constructed by insertion of DNA fragments from different species into a vector, which can be replicated in a bacterial host.