A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. [1][2][3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.

6 days ago · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in …

General information on each BAC (Bacterial Artificial Chromosome) clone collection is provided in this section. The table below provides an overview of the different BAC Clone Collections, source, coverage, and vector information.

The Human BAC Resource provides genome-wide resource of large-insert clones that helps integrate cytogenetic, radiation-hybrid, linkage, and sequence maps of the human genome.

Jul 24, 2024 · Explore the structure, cloning process, and genomic applications of bacterial artificial chromosomes in this comprehensive guide. Bacterial Artificial Chromosomes (BACs) …

Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome.

To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates.

Jun 28, 2007 · Bacterial artificial chromosome (BAC) libraries are the large DNA insert libraries of choice and valuable tools for the map-based cloning of target quantitative trait loci, physical...

Jan 16, 2025 · Bacterial artificial chromosomes (BACs) are designed to replicate and maintain large DNA fragments within bacterial cells, typically Escherichia coli. This process involves several critical steps, each leveraging the unique structure and functionality of BAC vectors.

Jan 1, 2001 · Bacterial Artificial Chromosome (BAC) clones are a standard clone type for large scale genomic projects because of their high stability and large insert sizes (80–300 kb) (1 – 3).