All HCR™ Amplifiers (e.g., B1, B2, ...) are engineered to identical performance targets, so there is no reason to choose one system over another. If you are planning to multiplex several targets, then each target should use a different HCR™ Amplifier paired with a …

An HCR™ Amplifier comprises two kinetically trapped reporter-labeled hairpins, h1 and h2, that co-exist metastably on lab time scales. Hairpins h1 and h2 store the energy to drive a conditional self-assembly cascade upon exposure to initiator i1.

HCR™ IF + HCR™ RNA-FISHenables a unified approach to multiplexed, quantitative, high-resolution pro- tein immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH), with quantitative 1-

Repeat HCR (R-HCR) provided significantly higher sensitivity for detection of repeats and allowed accurate tracking of endogenous GGGGCC repeat RNAs in patient cells in response to cellular stress activation.

Using multiple initiator-specific DNA hairpins tagged with different fluorophores (e.g., B1H1-AF647:B1H2-AF647; B2H1-AF488:B2H2-AF488), we were able to detect and directly compare the HCR signal of the same gene in different laser channels.

In situ hybridization based on the mechanism of the hybridization chain reaction (HCR) has addressed multi-decade challenges that impeded imaging of mRNA expression in diverse organisms, offering a unique combination of multiplexing, quantitation, sensitivity, resolution and …

Dec 12, 2020 · HyPR-seq involves hybridizing DNA probes to RNA, distributing cells into nanoliter droplets, amplifying the probes with PCR, and sequencing the amplicons to quantify the expression of chosen genes.

Oct 18, 2022 · In short, gene expression (via in situ HCR 3.0), cell proliferation (via EdU labelling), proteins (via Immunohistochemistry) and nuclei (DAPI/Hoechst staining) can be assessed in whole-mount tissue samples, allowing the co-visualisation of various types of molecules in the same specimen.

HCR probes Sequence (part of probe homologous to transcripts of interest) Amplifier ... lacZ B1H1, B1H2 Alexa Fluor 546 lacZ B1H1, B1H2 Alexa Fluor 488 prgB B2H1, B2H2 Alexa Fluor 488 . Title:

Jan 15, 2024 · Herein, a novel functional DNA -linked immunosorbent assay (DLISA) with dual-modality based on hybrid chain reaction (HCR) has been successfully developed for ultrasensitive detection of AFB1. The strategy relies on AFB1 immune-bridged occurrence of HCR and the salt-induced aggregation of gold nanoparticles (AuNPs).