Atg7 under-wing and NTD-CTD junction regions interact with the E2 core domain backsides and edges of Atg3 and Atg10, with unique insertions extending from Atg3 (flexible region) and Atg10 (β-hairpin) interacting with the Atg7 shoulder groove.

Mar 29, 2024 · Atg7 is an essential autophagy-related gene that encodes a series of proteins of ~630–700 residues in length found in various eukaryotes 4. ATG7 is a homodimeric E1 enzyme that drives ATG8 and...

Nov 11, 2012 · Crystallographic and mutational analyses of yeast (Atg7–Atg3) 2 and (Atg7–Atg10) 2 complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain...

In this review, R. Taylor, J. Collier & colleagues discuss the biological functions of the autophagy protein ATG7, and the impact of its impairment on signalling pathways and human pathology.

Feb 6, 2013 · In our recent study, we used a chemical crosslinking approach to enable crystallization and structure determination for S. cerevisiae Atg7-Atg3 and Atg7-Atg10 complexes with E1 and E2 enzyme active site cysteine residues juxtaposed—as required for catalysis of …

Aug 1, 2019 · CgATG10 might be involved in oyster innate immune response by regulating the autophagosome formation of autophagy, which could inhibit the expression of CgIRF-1 and negatively regulate the interferon system during anti-virus immune response. These results would provide referential information about the autophagy process in bivalves, and also be ...

Jan 8, 2018 · Firstly, the E1-like and E2-like enzymes Atg7 and Atg10 constitutively conjugate Atg5 to Atg12 through a glycyl lysine isopeptide bond 4. The covalently bound Atg5 and Atg12 then acts as an...

In our recent study, we used a chemical crosslinking approach to enable crystalli-zation and structure determination for S. cerevisiae Atg7-Atg3 and Atg7-Atg10 com-plexes with E1 and E2 enzyme active site cysteine residues juxtaposed—as required for catalysis of UBL transfer.

Sep 15, 2016 · Combination analysis of ULK1, Beclin 1, ATG3, ATG10 revealed the improved prognostic accuracy for gastric cancer. Our study showed that ATG proteins might serve as novel prognostic biomarkers in gastric cancer, and supply a new valuable insight into cancer treatment targeting autophagy for patients.

Aug 27, 2012 · Crystallographic and mutational analyses of yeast (Atg7-Atg3)(2) and (Atg7-Atg10)(2) complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain recruits distinctive elements from the Atg3 and Atg10 'backsides'.