Apr 1, 2024 · Our findings suggest that KAT5 overexpression inhibits NLRP3-mediated cardiomyocyte pyroptosis to relieve MIRI through modulation of STUB1/LATS2/YAP/β-catenin axis, providing a potential...

Senescence associated β-galactosidase (Sigma) staining was performed following the manufacturer's protocol. Briefly, AC16 cells were treated with 0.1 µM doxorubicin or vehicle for 24 hours, fixed overnight at 37 °C, then imaged at 20× on …

Apr 1, 2022 · Organized sarcomeric structure is detectable in HL-1 cells but not in H9C2 or AC16. Differentiation of AC16 or H9C2 induces minor changes towards cardiac phenotype. Functional tests reveal the restricted responsiveness of all the cardiac cell lines. This is the first systematic study showing major limitations of cardiac cell lines.

Aug 16, 2024 · Co-immunoprecipitation (coIP) and double-fluorescence staining confirmed IFI-16’s ability to interact directly with galectin-3. These findings underscore IFI-16’s critical role as a pro-inflammatory factor in post-MI MR, suggesting its inhibition as a potential therapeutic strategy.

5 days ago · b, Immunofluorescence staining of AC16 cardiomyocytes transiently transfected with a plasmid encoding TMEM65-Myc-FLAG or empty vector control, corresponding to experiment shown in Fig. 1f. Data ...

Silencing PTPN4 reverses the inhibitory effect of knockdown of miR-16-5p on H/R-induced AC16 cell damage: (a) PYPN4 protein in AC16 cells; (b) LDH levels in AC16 cells; (c) CK-MB levels in AC16 cells; (d) AC16 cell apoptosis level; (e) autophagy-related protein levels in AC16 cells.

May 17, 2024 · (E) FerroOrange staining in live CYRAB-overexpressed AC16 cells under erastin treatment. The limb-bud and heart-αB-crystallin axis affects p53 signaling via both protein-protein interactions with p -αB-crystallin and p53 transcriptional regulation

The present study aimed to identify differentially expressed genes in hypoxic human AC16 cardiomyocytes using next-generation sequencing and bioinformatics. A total of 24 genes (15 upregulated and 9 downregulated) with potential micro (mi)RNA-mRNA interactions were identified in the miRmap database.

Jan 1, 2018 · TUNEL staining was performed to evaluate DNA damage in AC16 cells. HE and Masson staining were performed to detect the morphology and degree of fibrosis in myocardium from mice. Immunohistochemical staining, immunofluorescence staining, western blot and real-time PCR were used to detect the protein and mRNA expression of caspase-1, IL-1β and ...

Cytoskeleton stained by β-TUBULIN and nuclear stained DAPI of AC 16 cells after the treatment of the three drugs; green: cytoskeleton, blue: nuclear (D). The data are presented as the mean...