Protocol to quantify palmitoylation of cysteines in budding yeast
Palmitoylated proteins: purification and identification
Jun 21, 2007 · ABE comprises a sequence of three chemical steps: (i) an exhaustive blockade of free thiols with N -ethylmaleimide (NEM); (ii) hydroxylamine treatment to release thioester …
- Author: Junmei Wan, Amy F Roth, Aaron O Bailey, Aaron O Bailey, Nicholas G Davis
- Publish Year: 2007
Detection of protein palmitoylation in cultured hippocampal …
Feb 18, 2013 · Development of the Acyl-Biotin Exchange (ABE) assay enables more rapid and high sensitivity detection of palmitoylated proteins, and is optimal for measuring the dynamic …
- Author: G. Stefano Brigidi, Shernaz X Bamji
- Publish Year: 2013
- Studies of Abe Assay
Analysis of the brain palmitoyl-proteome using both …
Jun 12, 2017 · Three techniques have emerged more recently for purifying all palmitoylated proteins from extracts such as cell lysates or tissue …
- Author: Matthew J. Edmonds, Bethany Geary, Mary K. Doherty, Alan Morgan
- Publish Year: 2017
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative …
Here, we describe an innovative biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest and …
Detection of Protein Palmitoylation in Cultured …
The IP-ABE assay presented here is a simple and highly sensitive method for detecting palmitoylated proteins in cultured primary hippocampal neurons, using mostly standard biochemical techniques. This makes the assay easily …
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Protocol for a semi-quantitative approach to identify protein S ...
Jun 21, 2024 · Images depicting some of the steps of the acyl-biotin exchange (ABE) assay (A) Protein precipitation: clear layers can be observed, with the upper phase (metabolites, salts, …
A Simple, Semi-Quantitative Acyl Biotin Exchange-Based Method …
Mar 21, 2023 · With respect to the conventional ABE-based protocol, we optimized and validated a rapid semi-quantitative assay that is shown to be significantly more sensitive and highly …
Frontiers | Chemical probe mediated visualization of …
Feb 20, 2023 · The most common method for detecting palmitoylated proteins without metabolic labelling is through chemical labeling of palmitoylated cysteines with the acyl-biotinyl exchange (ABE) assay. We have adapted the ABE assay …
Palmitoylation of SARS-CoV-2 S protein is essential for viral ...
Jun 11, 2021 · To explore the palmitoylation of SARS-CoV-2 S protein, we performed an acyl-biotin exchange (ABE) assay. 4 As shown in Fig. 1a, ectopically expressed S protein was …
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