May 16, 2022 · Cryo-EM took hold in AAV structure determination after many of the canonical natural serotypes had been solved crystallographically, and as attention was moving toward …

1 day ago · The structures of several AAV capsids have been determined by X-ray crystallography and/or cryo-electron microscopy (cryo-EM) 8,9,10. All AAV capsids VPs …

To understand the mechanisms underlying AAV Rep function, we investigated the cryo-EM and X-ray structures of Rep68–ssDNA complexes. Surprisingly, Rep68 generates hybrid ring …

Cryo-EM is the only technique that allows for the intuitive and rapid imaging of rAAV particles in a near-native state. This capability provides highly convincing data for characterizing rAAV …

In this white paper, we show that cryo-EM has two important applications in the development of AAV therapies. First, we demonstrate how simple 2D cryo-EM imaging can support the …

Oct 25, 2021 · High-resolution cryogenic electron microscopy (cryo-EM) structures detail adeno-associated virus (AAV) receptor protein binding interactions. AAV’s glycan 'primary receptors' …

Adeno-associated virus serotype 2 (AAV-2) is a leading candidate vector for gene therapy. Cell entry starts with attachment to a primary receptor, Heparan Sulfate Proteoglycan (HSPG) …

Sep 14, 2022 · AAV is now well-enough characterized that it has also become a model system for EM methods development. Heralding a new era, cryo-EM is now also being deployed as an …

In this study, we used two-dimensional class averages and three-dimensional classes, intermediate outputs in the single particle cryo-electron microscopy (cryo-EM) image …

Dec 26, 2022 · Adeno-associated virus (AAV) is the vector of choice for several approved gene-therapy treatments and is the basis for many ongoing clinical trials. Various strains of AAV …