Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Plasmid pGW03 AAV-TBG-Cre-sgRNA from Dr. Sidi Chen's lab is published in Cancer Discov. 2020 Dec;10 (12):1912-1933. doi: 10.1158/2159-8290.CD-19-1448. Epub 2020 Sep 4. This …

Jun 13, 2023 · CRISPR-based genetic screening directly in mammalian tissues in vivo is challenging due to the need for scalable, cell-type selective delivery and recovery of guide …

Mar 18, 2022 · We provide a protocol for gene editing in mouse hepatocytes in vivo using the CRISPR-Cas9 technology via AAV delivery. This protocol describes the construction of AAV …

We show that a single adeno-associated viral (AAV) vector containing a recombinase-dependent Staphylococcus aureus Cas9 (SaCas9) and a single guide RNA (sgRNA) are as efficient as …

Sep 18, 2020 · AAV-CRISPR/Cas9 permits gene mutagenesis in the adult CNS. Current methods determining In Vivo on-target mutagenesis have been limited by the ability to isolate virally …

We show that a single adeno-associated viral (AAV) vec-tor containing a recombinase-dependent. RNA (sgRNA) are as efficient as conventional condi-tional gene knockout and can be adapted …

Feb 8, 2021 · Three cell lines and two primary neuron cultures were used to validate the disruption of NeuN by single-guide RNAs (sgRNA) harboring distinct spacers and scaffold …

In the case of hepatocytes, we used an AAV8 vector containing Cre driven by the liver specific Thyroid Hormone-binding Globulin (TBG) promoter and single guide RNA (sgRNA) (Figure 1 …

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.