Aug 24, 2021 · To comprehensively analyze the effect of CHX on human cells, we focused on HEK 293T cells as a well-established model in ribosome profiling.

Jan 25, 2022 · Overexpression of USP44 significantly promoted the degradation of Ku80, and knockout of USP44 inhibited the degradation of endogenous and exogenous Ku80 through treatment with CHX, which means ...

Among the known inhibitors of eukaryotic translation is cycloheximide (CHX, 1), the most common laboratory reagent used to inhibit protein synthesis (Fig. 1). CHX has been shown to block the elongation phase of eukaryotic translation. It binds the …

Hence, we directly compared three treatments (Fig. 1a and Supplementary Fig. 1g): First, we incubated HEK 293T cells for 1 min in a medium containing CHX prior to lysis and included the drug in...

May 30, 2022 · We set out to investigate whether BAP1 functions in the repair of UV-induced DNA damage. BAP1 depletion rendered 293T cells hypersensitive to UV-C light (254 nm, hereafter denoted UV) (Fig. 1A,...

I am trying to detect cleaved caspase-3 by western blotting in 293T cells treated with TNF-α+CHX. Although I am able to detect procaspase-3 , I cannot detect a cleaved form of caspase-3.

Apr 23, 2021 · Wild-type H2B (H2BWT) and lysine residue-mutated H2B-transfected HEK293T cells were pretreated with cycloheximide (CHX), which blocks protein synthesis, for distinct time points.

Apr 8, 2014 · 2D confocal images of CHX inhibited HEK293T cells. HEK293T cells stimulated for 0, 1, 2 or 4 hours with CHX; showing DAPI (blue), β-catenin (green), N-cadherin (red) and 3 channel overlay.

Download scientific diagram | | Cycloheximide (CHX) chase assay for stably transfected HEK293 Endoglin WT and the two mutant variants P165L and V105D. (A-C) HEK293 stably transfected with p. ENG...

Sep 28, 2004 · We analyzed 30 clones from this group by individually transfecting them into 293T cells and determining the half-life by FACS-based analysis of CHX chase kinetics.