Plasmid pEGFP-N1-TFEB from Dr. Shawn Ferguson's lab contains the insert TFEB and is published in Sci Signal. 2012 Jun 12;5 (228):ra42. This plasmid is available through Addgene.

Aug 17, 2018 · Transcription factor EB (TFEB) is a member of the MiT-TFE helix–loop–helix leucine-zipper (bHLH-Zip) family of transcription factors and plays a pivotal role in organelle biogenesis and cell...

Jun 12, 2012 · Here, we identified an interaction between the lysosome-localized mechanistic target of rapamycin complex 1 (mTORC1) and the transcription factor TFEB (transcription factor EB), which promotes lysosome biogenesis.

Jul 11, 2018 · Here we found that TFEB has a regulated nuclear export signal (NES) in which phosphorylation at the ERK/mTORC1 phosphorylation site at S142 primed for phosphorylation by GSK3β at S138....

The master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. However, TFEBS211A localizes diffusely in both cytoplasm and nucleus and, as we show, ...

Mar 19, 2015 · We found that TFEB overexpression reduces the accumulation of aggregated α-syn by inducing autophagic clearance of α-syn. Furthermore, we showed that pharmacological activation of TFEB using 2-hydroxypropyl-β-cyclodextrin promotes autophagic clearance of aggregated α-syn.

Dec 26, 2024 · Repression in several TNBC models, including in patient-derived xenografts (PDXs), induces nuclear translocation of transcription factor EB (TFEB), which drives a transcriptional program that controls endolysosome function and exocytosis.

Plasmid pMRX-IP TFEB-sfGFP from Dr. Eisuke Itakura's lab contains the insert TFEB and is published in Sci Rep. 2019 Aug 12;9 (1):11635. doi: 10.1038/s41598-019-48131-2. This plasmid is available through Addgene.

Time-lapse imaging of cells stably expressing TFEB-GFP revealed that TFEB rapidly redistributes from the nucleus to the cytosol within 20 min upon nutrient replenishment (Fig. 1a), indicating that TFEB undergoes active nuclear export.

Here we found that TFEB has a regulated nuclear export signal (NES) in which phosphorylation at the ERK/mTORC1 phosphorylation site at S142 primed for phosphorylation by GSK3β at S138. Phosphorylation at both sites was required for efficient nuclear export and GSK3β was inhibited via AKT downstream from mTORC2 in response to glucose limitation.