Oct 14, 2018 · tdTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a somewhat slowly-maturing tandem dimer with low acid sensitivity.

Tandem dimer Tomato (tdTomato) provides a useful alternative to enhanced green fluorescent protein (eGFP) for performing simultaneous detection of fluorescent protein in histological sections together with fluorescence immunohistochemistry (IHC). eGFP has many properties that make it useful for cell …

About tdTomato. tdTomato is a tandem dimer fluorescent protein derived from the coral anemones species Discosoma sp. It has an excitation peak at 554 nm and an emission peak at 581 nm.

tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is as photostable as mCherry (Shaner et al. 2004).

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Our results show that the RBPMS CreERT2-tdTomato mouse line is a valuable tool for lineage tracing, disease modeling, drug screening, as well as isolating specific target cells. Subject areas: Natural sciences, Biological sciences, Physiology, Biological sciences research methodologies.

Nov 21, 2004 · The highest brightness (product of extinction coefficient, 138,000 M −1 cm −1, and quantum yield, 0.69) is found in tdTomato, at the cost of doubling the molecular weight.

Feb 20, 2025 · tdTomato is a red fluorescent protein with an excitation peak of 554nm and an emission peak of 581nm in vitro. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Discosoma drFP583 gene ( GenBank:AF168419 ; AAF03369), consisting of a tandem dimer of the dTomato fluorescent protein linked by a 12 residue ...

Jun 1, 2024 · Fluorescent proteins have been widely used to trace biological events in animal models. TdTomato expression led to unexpected retinal dysfunction and ultrastructural defects in the transgenic mouse retina. Retinal dysfunction was seen as the reduced photopic responses and lower contrast sensitivity.

Oct 10, 2008 · pCMV-tdTomato is designed for use as a marker for cotransfection or cell tracking. The red fluorescence of tdTomato can be detected by fluorescence microscopy, allowing direct visual . imaging. In addition, flow cytometry can be used to enrich transfected cell populations.