snRNA-seq, also known as single nucleus RNA sequencing, single nuclei RNA sequencing or sNuc-seq, is an RNA sequencing method for profiling gene expression in cells which are difficult to isolate, such as those from tissues that are archived or which are hard to be dissociated.

Dec 19, 2022 · Single-nucleus RNA sequencing (snRNA-seq) is an alternative or complementary approach for cells that are difficult to isolate. In this review, we will provide an overview of the experimental and analysis steps of snRNA-seq to understand the methods and characteristics of general and tissue-specific snRNA-seq data.

Single‐nucleus RNA sequencing (snRNA‐seq) is an alternative single‐cell sequencing method. Instead of sequencing all the mRNA in the cytoplasm of cells, scRNA‐seq only captures the mRNAs in the nucleus of cells.

May 11, 2020 · Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors. Each...

Single-nuclei RNA-seq (snRNA-seq) provides another strategy for performing single-cell transcriptomics where individual nuclei instead of cells are captured and sequenced. The major advantage of snRNA-seq over scRNA-seq is that the former does not require the preservation of cellular integrity during sample preparation, especially dissociation.

Dec 11, 2020 · We sought to gain insight into the range of myonuclear heterogeneity in mammals, through profiling of nuclei from mouse skeletal muscle using single-nucleus RNA-sequencing (snRNA-seq).

Apr 6, 2020 · Here, we directly compare seven methods for single-cell and/or single-nucleus profiling—selecting representative methods based on their usage and our expertise and resources to prepare...

Dec 28, 2023 · Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) are both efficient techniques for studying heterogeneity in the transcriptome of individual cells. While similar in principle, each technique has its own strengths that make it ideal for different applications.

基于单细胞测序的强大属性，其逐渐成为近两年生物及医学研究领域的热门工具，其中单细胞转录组测序（scRNA-seq）是应用最广泛的单细胞测序技术，在在科研领域得到大量应用。 但随着scRNA-seq应用的深入，其技术的局限性也逐渐凸显，例如对于上皮含量高、细胞外基质含量高的的实体组织，进行单细胞测序研究存在如下障碍，包括人为解离诱导的特定基因激活、酶解后与实际不成比例的细胞类型再现等。 总结下来主要是： scRNA-seq的局限性. 1、 仅适用于新鲜组 …

snRNA-Seq uses a mild and quick nuclear dissociation protocol to isolate and sequence RNA within the nucleus. The method minimizes technical issues that can arise from common dissociation protocols, especially in studying immediate early gene (IEG) behavior.