Apr 16, 2021 · Literature suggests that snRNA associates with a number of nuclear RNA-binding proteins, including MSUT2, SRRM2 and SFPQ, all of which have been shown previously to exhibit abnormal nuclear distribution in Alzheimer brain (Wheeler et al., 2019; Younas et al., 2020; Ke et al., 2012; Tanaka et al., 2018). These proteins are associated with ...

Oct 9, 2020 · Thrupp et al. propose that snRNA-Seq technology fails to recapitulate microglia activation states in humans because activation genes are underrepresented in non-diseased human brains by snRNA-Seq. The paper reveals a difference in sensitivity between scRNA-Seq and snRNA-Seq. This is an important observation that highlights a limitation of snRNA ...

Jun 22, 2023 · Comparing snRNA-Seq data from 108 people who carried one or two copies of APOE4 to transcriptomes of 251 who carried two copies of APOE3, Sun found 2,482 DEGs, of which just 4 percent overlapped with AD DEGs. Among the most downregulated were those involved in cell junction maintenance, transport of molecules across the BBB, and angiogenesis.

Oct 7, 2023 · Among the snRNA-Seq data, the scientists hunted for hybrid transcripts—those with segments derived from two different genes. Such transcripts signify gene-fusion events that result from sloppy repair of double-strand DNA breaks (image below). The researchers found a plethora of such gene fusions in excitatory neurons from people in late-stage AD.

Oct 19, 2020 · Thrupp et al. propose that snRNA-Seq technology fails to recapitulate microglia activation states in humans because activation genes are underrepresented in non-diseased human brains by snRNA-Seq. The paper reveals a difference in sensitivity between scRNA-Seq and snRNA-Seq. This is an important observation that highlights a limitation of snRNA ...

Jan 6, 2021 · Our data would have corroborated some of the conclusions reached by Olah et al., but also yielded some important differences that may be due to heterogeneity of patients analyzed, brain regions processed, or technical procedures used to perform snRNA-seq. Evaluation and discussion of these differences would have been helpful for advancing the ...

Mar 3, 2023 · Statistical analysis of the snRNA-Seq data suggested that, under disease conditions, homeostatic microglia transition toward either the cluster 3 profile or a classic disease-associated microglia profile (Jun 2017 news). Cluster 3 microglia may represent a protective, anti-inflammatory state, the authors suggested.

Dec 21, 2023 · Rather, they are amenable to any type of single-cell assay. As a proof-of-concept for spatial multiomic analysis, the researchers Slide-tagged a section of a metastatic melanoma sample, then used both snRNA- and ATAC-Seq to correlate gene expression with chromatin accessibility. Both were heterogeneous throughout the melanoma.

Oct 22, 2020 · Researchers use snRNA-Seq to get a granular picture of how gene expression changes in a given tissue. Previous studies on AD brains have shed light on neuronal and glial changes, but included few endothelial cells (May 2019 news; Nov 2019 news; Wang et al., 2020). Endothelial Burst. The e1, e3, and e4 subtypes of endothelial cells are ...

May 9, 2021 · snRNA-Seq of mouse choroid plexus finds inflammation goes up with age. In AD brain samples, their analysis found numerous gene expression changes compared to healthy brain, and confirmed a massive loss of vascular cell types, particularly those responsible for maintaining the extracellular matrix.