A single-chain antibody generation system yielding CAR-T …
Mar 2, 2021 · In this study, we have established a T-cell-based scFv generation system by exploiting CAR-library T cells. Second generation (CD28ζ) CAR-library T cells were stimulated …
Targeted delivery of a PD-1-blocking scFv by CAR-T cells ... - PubMed
We modified CAR-T cells to secrete PD-1-blocking single-chain variable fragments (scFv). These scFv-secreting CAR-T cells acted in both a paracrine and autocrine manner to improve the …
CAR-T design: Elements and their synergistic function
Aug 1, 2020 · scFv affinity is a key parameter that has been modulated to improve specificity of the CAR and reduce “on-target, off-tumor” side effects, which is of particular importance when …
Novel high‐affinity EGFRvIII‐specific chimeric antigen receptor T …
We have made novel chimeric antigen receptor T (CART) cells specific for glioblastoma, a devastating brain tumor with poor survival. This receptor is of extremely high affinity, and the …
Targeted delivery of a PD-1-blocking scFv by CAR-T cells ... - Nature
Oct 1, 2018 · These scFv-secreting CAR-T cells acted in both a paracrine and autocrine manner to improve the anti-tumor activity of CAR-T cells and bystander tumor-specific T cells in …
Identification of Potent CD19 scFv for CAR T Cells through scFv ...
Dec 1, 2020 · Most of these anti-CD19 CAR T cells use FMC63 single-chain variable fragments (scFvs) for binding CD19 expressed on the cancer cell surface. In this study, we screened …
Identification of Potent CD19 scFv for CAR T Cells through scFv ...
Most of these anti-CD19 CAR T cells use FMC63 single-chain variable fragments (scFvs) for binding CD19 expressed on the cancer cell surface. In this study, we screened several known …
Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv …
Jan 18, 2022 · These findings demonstrated the successfulness in creation of a new CAR T cells containing a novel fully human-derived scFv specific to CD19 + cancer cells. In vivo studies …
Enhancing CAR T Cell Anti-Tumor Efficacy through Secreted …
Dec 7, 2017 · Expression of PD-1-blocking scFv enhances CAR T cell function against PD-L1 expressing tumors in xenograft models of hematological and solid tumors by enhancing …
Effect of scFv Domain on the Function and Toxicity of CAR-T
Jul 15, 2021 · Most clinical CAR-T trials use scFvs from mouse antibodies, which increases the immunogenicity of CAR-T and may lead to toxicity or limit the persistence of CART cells. This …