Whereas semi-quantitative methods, such as Northern blotting analysis, allow only a dichotomous differentiation between positive and negative gene expressions, the real-time quantitative polymerase chain reaction (qRT-PCR) combines a large range of results with an accurate and highly reproducible quantitation of genes.

Jun 12, 2019 · Activation of the p16 Ink4a -associated senescence pathway during aging breaks muscle homeostasis and causes degenerative muscle disease by irreversibly dampening satellite cell (SC) self-renewal...

Aug 15, 2013 · Chromatin immunoprecipitation (ChIP) and subsequent qPCR (qChIP) analyses showed that AP4 occupies E-boxes present ∼ 4.5 kbp upstream and 400 bp downstream of the p16 transcriptional start site...

In this chapter, we will describe the extraction of total RNA from frozen tissue, the reverse transcription to cDNA, and the analysis of mRNA expression levels of the target gene p16 INK4a and the internal reference gene β-actin, using quantitative real-time PCR.

Aug 15, 2017 · A p16 RT-qPCR (p16-mRNA assay) was run on mRNA extracted from formalin-fixed paraffin-embedded sections. Two p16 immunohistochemistry (IHC) readings, a visual read by a histopathologist (Visual IHC) and a digital read of a high-resolution scan (Digital IHC), were done on adjacent sections.

Real-time quantitative polymerase chain reaction (qRT-PCR) is a powerful tool used for gene expression analysis, genotyping, pathogen detection, muta-tion screening, and DNA quantification.

Oct 31, 2013 · Expression of p16 INK4a mRNA was quantified by qPCR (standard curve method) using at least two independent RT reactions for each biopsy and the SensiMix TM SYBR Low ROX Kit (BIOLINE).

Aug 8, 2017 · A p16 RT‐qPCR (p16‐mRNA assay) was run on mRNA extracted from formalin‐fixed paraffin‐embedded sections. Two p16 immunohistochemistry (IHC) readings, a visual read by a histopathologist (Visual IHC) and a digital read of a high‐resolution scan (Digital IHC), were done on adjacent sections.

Dec 3, 2020 · The aim of this study was to measure the closeness of agreement of methylation values between pyrosequencing and nested MS-qPCR, analyzing the methylation status of p16/INK4A using cytological samples obtained by EBUS-TBNA in lung cancer patients.

Aug 31, 2023 · Here, using genetic and chemical approaches to manipulate senescent cells, we show that removal of p16 High cells resulted in the 4FR of somatic cells into totipotent-like stem cells.