Addgene: Vector Database - pUC8
Assembled from pUC18 and M13mp8 by F. Pfeiffer, MPI, Martinsried Hosts: E.coli JM83, E.coli JM105, E.coli JM109, E.coli NM522. Related vectors: pUC7, M13mp8, pUC9. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) How was your experience today? Addgene is a nonprofit plasmid repository.
Physical maps of plasmids pUC8 and pUC8:15. pUC8 is a …
Download scientific diagram | Physical maps of plasmids pUC8 and pUC8:15. pUC8 is a derivative of plasmid pBR322 and filamentous phage M13.
pUC8 mouse IFN-gamma - Addgene
Plasmid pUC8 mouse IFN-gamma from Dr. Howard Young's lab contains the insert interferon-gamma. This plasmid is available through Addgene.
pUC8-SZ - Addgene
Plasmid pUC8-SZ from Dr. Takeshi Sano's lab contains the insert streptavidin and is published in Nucleic Acids Res. 1986 Feb 25. 14 (4):1871-82. This plasmid is available through Addgene.
Understanding the pUC8 Plasmid Vector - Genspark
Jan 12, 2025 · pUC8 is a derivative of the plasmid pBR322 and filamentous phage M13, designed for cloning and expression in E. coli. It is a high copy number plasmid, with a copy number ranging from 500 to 700, due to a mutation in its origin of replication.
The pUC plasmids, an M13mp7-derived system for ... - ScienceDirect
Oct 1, 1982 · The resulting plasmids pUC8 and pUC9 allow one to clone doubly digested restriction fragments separately with both orientations in respect to the lac promoter. The terminal sequences of any DNA cloned in these plasmids can be …
The size of the pUC8/EcoRI linear DNA fragment, in base pairs, is ~2700. A sample volume of 18-20 μl is recommended for ethidium bromide staining and 35-38 μl is optimal for FlashBlueTM or InstaStain® Blue staining. Shortly before loading, heat the fragment at 65° C for 3 minutes and quickly chill on ice.
The pUC plasmids, an M13mp7-derived system for insertion
The resulting plasmids pUC8 and pUC9 allow one to clone doubly digested restriction fragments separately with both orientations in respect to the lac promoter. The terminal sequences of any DNA cloned in these plasmids can be characterized using the universal M13 primers.
Properties of pUC8- 1) High copy number- During construction of pUC8, there was chance mutation within origin of replication that results in having a copy no. of 500-700 even before replication. 2) Size- It is 2750 bp in length, and cloning vector need to be less than 10kb. Due to …
Construction of a family of universal expression plasmid vectors
A family of universal expression vectors based on the pUC8 and pUC9 plasmids has been constructed. These vectors cover all three possible reading frames in both directions, allowing any synthetic DNA, genomic DNA or cDNA to be expressed under control of the lac promoter.