Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, …

pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). NEB offers a selection of …

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections. Storage DNA can be stored at …

If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal, then colonies will appear blue, as the plasmid encodes for the α-peptide required to make …

Aug 3, 2023 · It is a commonly used cloning vector in the bacteria E. coli. pUC19 is 2686 bp in length. The molecular weight of the pUC19 vector is 1.75×10 6 Da. It is a small plasmid with a …

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in …

pUC19 is a small, high-copy number E. coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). It con-tains the pMB1 origin of replication from pBR322, but it lacks the rop …

Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

Plasmid DNA from E. coli RRI pUC19, buffered aqueous solution; CAS Number: 9007-49-2 at Sigma-Aldrich ... The pUC19 plasmid (2,686 bp) confers ampicillin resistance and complement …

Oct 26, 2024 · The pUC19 cloning vector is a compact plasmid, approximately 2.7 kilobases in length, designed for efficient DNA manipulation. Central to its design is the multiple cloning …