Jun 23, 2020 · ERK1 and ERK2 are part of a family of structurally related kinases, called mitogen-activated protein kinases (MAPKs), the signalling mechanism of which depends on an activating phosphorylation...

Mar 1, 2025 · To address these limitations, we developed enhanced KTRs (eKTRs) for PKA and extracellular signal–regulated kinase (ERK) by (i) increasing KTR size, which reduces the confounding effect of KTR diffusion through the nuclear pore and (ii) modulating the strength of the bipartite nuclear localization signal in their kinase sensor domains, to ensure...

Oct 26, 2022 · Here we investigated how spatially organized β 2 -adrenergic receptor (β 2 AR) signalling controls ERK. Using subcellularly targeted ERK activity biosensors 5, we show that β 2 AR signalling...

Jun 2, 2014 · In vivo FRET demonstrates that ERK positively regulates the neutrophil recruitment cascade in the intestine by promoting adhesion and migration. Many chemical mediators regulate neutrophil recruitment to inflammatory sites.

Apr 1, 2020 · PKA regulates ERK activation by phosphorylation of several proteins in the ERK activation pathway, including Src, Rap1GDP, C-Raf and B-Raf, and can either decrease or increase ERK activation. PKA phosphorylates a set of non-receptor tyrosine kinases called Src Family Kinases (SFK), of which Src is the prototype.

Jan 20, 2017 · Using a single excitation wavelength dual colour FLIM method we are able to detect fluorescence lifetime images of two donors to simultaneously measure PKA and ERK1&2 kinase activities in the...

Oct 1, 1998 · Here, we report that extracellular signal–related protein kinase (ERK) signaling is obligatory for Ca 2+ -stimulated transcription in PC12 cells and hippocampal neurons. The sequential activation of ERK and Rsk2 by Ca 2+ leads to …

4 days ago · The cerebral cortex, the anatomical foundation of human intelligence, underpins our advanced cognition and language. Deciphering the regulatory mechanisms driving its expansion in neuronal number and surface area is essential for elucidating the unique features of the human brain. Here, we demonstrate that ERK and PKA signaling pathways coordinately maintain the neurogenic identity and lineage ...

Mar 1, 2011 · Kinase signaling networks are often organized by scaffolding and anchoring proteins that help shape the dynamics of signal processing. AKAP-Lbc associates with the ERK scaffold protein KSR-1 to organize a growth factor and cAMP responsive signaling network.

We developed a dynamical model to study the effects of phosphodiesterase 4 (PDE4), a cAMP phosphodiesterase that is phosphorylated and inhibited by ERK, on the membrane insertion of AMPAR. The model predicted that PKA could be a downstream effector of ERK in regulating AMPAR insertion.