Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to glow green under UV light.

Bio-Rad’s pGLO Bacterial Transformation and GFP Kits offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light.

Jan 1, 2019 · Here, I describe some extensions of the pGLO transformation experiment that can be used to explore aspects of the system in more detail and to demonstrate other biological concepts. A key feature of the pGLO system is the interaction between the sugar L-arabinose and the AraC protein.

Lab report on E. Coli transformation using pGLO, detailing methods, results, and transformation efficiency. High school biology experiment.

In this lab you will perform a procedure known as genetic transformation. Genetic transformation literally means “change caused by genes,” and involves the insertion of a gene into an organism in in order to change the organism’s trait. Genetic transformation is …

In this lab, you will be using non-pathogenic E. coli . bacteria and pGLO, a plasmid modified with three genes. The pGLO plasmid contains the genetic codes for (see Table 2): 1. a green fluorescent protein (GFP) from the bioluminescent jellyfish, Aequorea victoria 2. ampicillin resistance (amp. R)

In the lab, bacterial transformation is a common method for producing more copies of genes (cloning) for insertion of that gene into a variety of model organisms. Expressing genes in model organisms can help determine the function of those genes in more complex organisms.

Scientists can use a process called genetic engineering to insert genes coding for new traits into a plasmid. In this lab, the pGLO plasmid has the GFP gene that codes for the green fluorescent protein and a gene that codes for a protein that gives the bacteria resistance to an antibiotic.