Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

Teach the central dogma and genetic engineering using E. coli and a gene from the bioluminescent jellyfish Aequorea victoria. This classic pGLO kit follows the same procedure used by molecular biologists to create "designer proteins".

Jan 1, 2019 · Here, I describe some extensions of the pGLO transformation experiment that can be used to explore aspects of the system in more detail and to demonstrate other biological concepts. A key feature of the pGLO system is the interaction between the sugar L-arabinose and the AraC protein.

The features of the pGLO plasmid are illustrated in the plasmid map below. Specific sequences, including gene and promoter sequences, are shown as thick lines along the circle with arrowheads that point in the direction of operation or transcription.

Bacterial transformation plasmid for regulated expression of GFP. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Efficient cleavage requires at …

Lab report on E. Coli transformation using pGLO, detailing methods, results, and transformation efficiency. High school biology experiment.

The pGLO plasmid (see image below) contains the GFP gene, the bla gene, the araC gene, the ori, the pBAD promoter, and multiple cloning sites. The GFP gene codes for the production of GFP. The Bla gene codes for beta-lactamase and enzyme which breaks down …

Bio-Rad’s pGLO plasmid has three special genes: one for GFP, a gene for antibiotic resistance, and a gene regulation system. This system can be used to control when the bacteria produce fluorescent protein.