Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b(+) or pET-28c(+).

pET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins …

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at …

Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information

Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information

Basic Vector Information. An e.coli expression vector pET-28a fused with N-His, N-Thrombin, and N-sumo. Designed for Ni-NIA affinity purification and tag removal.

Nco l Xba l T7 terminator pET-28a(+) 5.4kb L a c l K a n R p B R 3 2 2 O r i f 1 O r i Blp l Bgl ll Thrombin His tag RBS Xho l Not l Hind lll Sal l Sac l EcoR l BamH l Nde l

Provide PET-28a(+)-16H-3Xflag vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Aug 16, 2011 · The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown …

Download scientific diagram | Vector map of pET28a(+). from publication: Cloning, Expression and Purification of Plasmodium falciparum Protein Kinase 2 In Partial Fulfillment of the...