pET-28a(+) DNA - Novagen The Novagen pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.

Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets. PaeR7I does not recognize the sequence CTCTCGAG.

Novagen′s pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Synonym(s) : cloning vector, expression vector, pET Plasmid, vector

pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. 1. Centrifuge at 5,000×g for 5 min. 2.

The pET-28a-c (+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence. The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a …

The pET-28a-c(+) vectors carry an N-terminal HIS TAG ® /thrombin/T7•Tag configuration plus an optional C-terminal HIS TAG sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB074).

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.

pET-28a (+) DNA - Novagen The Novagen pET-28a-c (+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.

Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at …