Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

Feb 12, 2018 · The results of the gel electrophoresis of the isolated and amplified DNA samples with Polymerase Chain Reaction (PCR) technology resulted in the appearance of one band for each of the hairy...

Agarose gel electrophoresis of pET-gel and pET-28a(+) plasmids cleaved by double enzymatic hydrolysis. (1) DNA marker; (2) pET-gel digested with both SalI and NdeI; (3) Vector pET28a...

Oct 5, 2021 · Experimental Aim: To digest the plasmid DNA using EcoRI and HindIII and run the digested fragments on a 1% agarose gel. Observations: Obtained 2 prominent bands between 5-6 kb in both digestions.

Agarose gel electrophoresis for vector (pET28a) and insert (HMPREF0351_11084) after digestion with NcoI and EcoRI. L: DNA ladder, V: vector and I: insert. The current study re-configured the...

pET-28a Plasmid DNA is a versatile cloning vector that allows for efficient expression and purification of recombinant proteins in E. coli. Its T7 promoter system, 6xHis-tag, and Kanamycin resistance gene provide strong transcription,

·Gel Extraction of DNA Bands: pET28a/EC2.4.1.5/EC2.4.1.9. Separate the target DNA fragment from other fragments via agarose gel electrophoresis. Using a scalpel, excise the agarose gel slice containing the target DNA and place it into a 1.5 mL microcentrifuge tube.

The pET28a-NF-L is designed for protein expression by Infusion recombination. First, we amplified NF-L fragment by high-fidelity PCR with a length of 1059 bp. Then, we used restriction endonuclease EcoRI and XhoI to linearize the pET-28a vector backbone.

Gel Extraction of the Digested Bands: pET28a/EC2.4.1.5/EC2.4.1.9 > Transformation T4 DNA ligase-mediated ligation of pET28a-2.4.1.5 and pET28a-2.4.1.9

pET-28a(+) DNA - Novagen The Novagen pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Sign In to View Organizational & Contract Pricing