Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence Author: MilliporeSigma …

pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. 1. Centrifuge at 5,000×g for 5 min. 2.

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!

Patrick Shilling et al. increase the protein production yield from the pET28a expression plasmid by modifying the genetic modules that control transcription and translation initiation. These improved designs are applicable to most vectors in the pET series and can be easily implemented.

pET-28a (+) DNA - Novagen The Novagen pET-28a-c (+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. - Find …

Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker. Midwest Center for Structural Genomics.

Aug 16, 2011 · The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.

All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration …