Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure

Provide pET-28a(+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.

Plasmid pET28a (+)-SUMO-A' from Dr. Tom de Greef's lab contains the insert SUMO-A' and is published in Nat Commun. 2023 Nov 2;14 (1):7001. doi: 10.1038/s41467-023-42810-5. This plasmid is available through Addgene.

Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information

Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure

Aug 16, 2011 · The pET-28a(+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.

Provide pET-28b(+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.

pET28 - Free download as PDF File (.pdf), Text File (.txt) or read online for free. The document describes pET-28a-c(+) vectors for protein expression and tagging. The vectors carry an N-terminal His-Tag/thrombin/T7-Tag configuration and optional C-terminal His-Tag.