First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene. Sequence Author: New England Biolabs. Explore Over 2.7k …

pBR322 DNA is a common plasmid cloning vector in E. coli that is 4,361bp and contains ampicillin and tetracycline resistance genes.

pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco , it was named …

pBR322 is an E. coli plasmid cloning vector containing the origin of replication from pMB1 (a plasmid in the ColE1 com-patibility group; 1–3). The rop gene product, which regulates …

Aug 3, 2023 · pBR322 is a commonly used cloning vector in E. coli and has tremendous applications in cloning. Origin of replication, Restriction enzyme sites, Selectable marker sites.

The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA …

The plasmid contents of these strains were compared to that of pBAD322S, a 5535 bp spectinomycin-dependent vector that carries the wild type pBR322 origin and is of a size quite …

pBR322 is 4361 bp in length and contains: • The replicon rep responsible for the replication of plasmid (source – plasmid pMB1) • The rop gene, coding for the Rop protein, which promotes …

Provide pBR322 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

pBR322 is a well characterized plasmid cloning vector containing two antibiotic resistance genes, ampicillin (Amp) and tetracycline (Tet), and several convenient restriction endonuclease sites …