pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."

First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene. Sequence Author: New England Biolabs. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. …

Aug 3, 2023 · pBR322 is a commonly used cloning vector in E. coli and has tremendous applications in cloning. It was constructed in 1977 in the lab of Herbert Boyer at The University of California in San Francisco. It is a synthetic plasmid and was the first artificial plasmid to be constructed and used as a cloning vector.

pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.

pBR322 is an E. coli plasmid cloning vector containing the origin of replication from pMB1 (a plasmid in the ColE1 com-patibility group; 1–3). The rop gene product, which regulates plasmid replication by stabilizing the interaction between RNAI and RNAII transcripts, maintains the copy number at about 20 per cell.

Plasmid vector pBR322 is a well-established multipurpose cloning vector in laboratories worldwide, and a large number of derivatives have been created for specific applications and research purposes, including gene expression in its natural host, E. coli, and few other bacteria.

Jul 5, 2024 · At its core, pBR322 is a circular double-stranded DNA molecule, approximately 4,361 base pairs in length. This compact size makes it an ideal vector for cloning, as it can be easily manipulated and introduced into host cells.

The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA molecules in Escherichia coli.

May 12, 2023 · The pBR322 vector is used for a wide range of applications in molecular biology and genetic engineering, including cloning, gene expression, site-directed mutagenesis, and antibiotic resistance selection.

The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI.