In molecular genetics, an untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA.

In molecular genetics, the three prime untranslated region (3′-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon. The 3′-UTR often contains regulatory regions that post-transcriptionally influence gene expression.

Our approach to enhancing mRNA stability diverges from a conventional high-throughput screening strategy on mRNA UTR sequences, which is often laborious and costly, and instead offers a novel rational design strategy for RNA sequence optimization.

Oct 1, 2019 · 3' untranslated regions (3' UTRs) of messenger RNAs (mRNAs) are best known to regulate mRNA-based processes, such as mRNA localization, mRNA stability, and translation.

Mar 18, 2025 · The 5′ untranslated region (5’UTR), located upstream of the coding sequence, affects multiple aspects of mRNA function, influencing post-transcriptional gene expression. Understanding the 5’UTR’s role is essential for deciphering mechanisms that control protein synthesis and RNA stability.

Mar 20, 2025 · The untranslated regions (UTRs) influence important parameters like mRNA stability and translation efficiency. This study adopted a combinatorial screening strategy to enhance exogenous mRNA translation efficiency by de novo designing 5′UTRs and combining them with multiple potential 3′UTRs.

Jun 20, 2024 · Here, we design 5’UTRs for efficient mRNA translation using deep learning. We perform polysome profiling of fully or partially randomized 5’UTR libraries in three cell types and find that UTR...

Apr 5, 2024 · In this study, we adopted the principled approach of a language model to extract meaningful semantic representations from UTRs of raw mRNA sequences and further map them to predict functions of...

Modulation of untranslated regions (UTR) represents one approach to enhance both mRNA stability and translation efficiency. The current studies describe and validate screening methods using a diverse set of 5'UTR and 3'UTR combinations for improved expression of the Arginase 1 (ARG1) protein, a potential therapeutic mRNA target.

In this Review, we discuss the current mechanistic understanding of structures in 5′ untranslated regions (UTRs) of eukaryotic mRNAs and the emerging methodologies used to explore them.