Proteins and peptides that have an affinity for metal ions can be separated using metal chelate affinity chromatography. The metals are immobilized onto a chromatographic medium by …

IMAC is a widely-used method for rapidly purifying polyhistidine affinity-tagged proteins, resulting in 100-fold enrichments in a single purification step. The chelators most commonly used as …

Purification using the polyhistidine tag can be performed under either native or denaturing conditions by IMAC. The use of mild buffer conditions and imidazole as the elutant often yields …

Protein purification based on fusing peptide affinity tag to recombinant protein is widely preferred due to its ease of use. Immobilized Metal Ion Affinity Chromatography (IMAC) was …

We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of …

Immobilized metal affinity chromatography (IMAC) is the most utilized technique to specifically separate proteins upon the formed stable, reversible coordination bonds between metal ions …

Oct 11, 2020 · Immobilized metal affinity chromatography (IMAC) is a technique primarily used in research and development laboratories to purify proteins containing engineered histidine tags.

Feb 18, 2025 · Optimize your IMAC purification process with the right choice of metal ions and chelators. Learn how Ni2+, Co2+, and Zn2+ impact His-tagged protein puri...

IMAC Sepharose ® High Performance is supplied free of metal ions, enabling it to be used across a range of applications for purifying histidine-tagged as well as native proteins. It is available in …

IMAC is a powerful and versatile protein purification technique used to purify proteins, peptides, and other biomolecules that have an affinity for metal ions. Proteins containing amino acids …