Nov 15, 2017 · Deoxyribonucleoside triphosphates (dNTPs) are used in DNA synthesis and repair. Even slight imbalances can have adverse biological effects. This study validates a fast and sensitive HPLC–MS/MS method for direct quantification of intracellular dNTPs from tissue.

Jan 25, 2003 · The use of HPLC for determining cellular NTP and dNTP pool sizes has proven to be one of the most simple and rapid methods of measuring all of the NTPs and dNTPs at the same time.

Feb 22, 2022 · Information about the cellular concentrations of deoxyribonucleoside triphosphates (dNTPs) is instrumental for mechanistic studies of DNA replication and for understanding diseases caused by defects in dNTP metabolism. The dNTPs are measured by methods based on either HPLC or DNA polymerization.

Nov 25, 2021 · Information about the cellular concentrations of deoxyribonucleoside triphosphates (dNTPs) is instrumental for mechanistic studies of DNA replication and for understanding diseases caused by defects in dNTP metabolism. The dNTPs are measured by methods based on either HPLC or DNA polymerization.

Methods: A HPLC-MS/MS system was used to quantify intracellular NTP and dNTP pools. Results: The assay was linear between 50 nM, the lower limit of quantification (LLOQ), and 10 muM in cell lysate.

Deoxyribonucleoside triphosphates (dNTPs) are used in DNA synthesis and repair. Even slight imbalances can have adverse biological effects. This study validates a fast and sensitive HPLC–MS/MS method for direct quantification of intracellular dNTPs from tissue.

Dec 28, 1984 · Multiple cycles of high-performance liquid chromatography (HPLC) are therefore used to prepare ultrapure dNTPs for use in the electrophoretic assay of misincorporation.

A simplified procedure for the separation of both ribonucleoside triphosphates (NTP) and deoxyribonucleoside triphosphates (dNTP) is presented which utilizes reversed phase high-performance liquid chromatography coupled with diode array detection.

Methods based on HPLC separation followed by ultraviolet (UV) or mass spectrometry (MS, MS/MS) detection have been employed to resolve dNTPs (dATP, dGTP, dCTP, and dTTP) and ribonucleoside triphosphates (rNTPs; ATP, GTP, CTP, and UTP) (8 – 11).

May 5, 2020 · Cells maintain a fine-tuned, dynamic concentration balance in the pool of deoxyribonucleoside 5′-triphosphates (dNTPs). This balance is essential for physiological processes including cell cycle control or antiviral defense. Its perturbation results in increased mutation frequencies, replication arrest and may promote cancer development.