Cryogenic electron microscopy (cryo-EM) is a transmission electron microscopy technique applied to samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice.

Jun 22, 2022 · Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components...

Apr 3, 2024 · Here we discuss how the interplay between cryo-EM and cryo-electron tomography, as a connecting bridge to visualize macromolecules in situ, holds great promise to create comprehensive...

Mar 13, 2025 · We use cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers, and ROOL and GOLLD forming hexameric, octameric and dodecameric nanostructures, at 2.6 to 4.6 Å resolutions.

Jan 10, 2024 · We introduce TEMPy-ReFF, a method for atomic structure refinement in cryo-EM density maps. Our method represents atomic positions as components of a Gaussian mixture model, utilising their...

In this article, the principle, characteristics, history, current situation, workflow, and common problems of cryo‐EM are systematically reviewed. In addition, the new development direction of cryo‐EM—cryo‐electron tomography (cryo‐ET), is discussed in detail.

Using cryo-electron microscopy (cryo-EM) and chemical cross-linking mass spectrometry (CXMS), we solved the structure of the Saccharomyces cerevisiae exocyst complex at an average resolution of 4.4 Å.

Jan 20, 2022 · This review summarizes the current state of methods and results achievable by cryo-electron microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to understand what is required and how it might help to address their research questions.

Aug 18, 2022 · Two groups resolved cryo–electron microscopy (cryo-EM) structures of the intact BCR complex, which comprises a membrane-bound immunoglobin (Ig) linked to a transmembrane Ig-α/Ig-β (CD79) signaling complex (see the Perspective by Tolar and Pierce).

Mar 27, 2025 · Enhancing cryogenic electron microscopy (cryo-EM) 3D density maps at intermediate resolution (4-8 Å) is crucial in protein structure determination. Recent advances in deep learning have led to the development of automated approaches for enhancing experimental cryo-EM density maps. Yet, these methods are not optimized for intermediate-resolution maps and rely on map density features alone. To ...