Jun 11, 2024 · Burkitt’s lymphoma: 90% have translocation of c-myc or variants; translocation causes continuous stimulation by adjacent enhancer element of immunoglobulin gene or mutations in myc gene regulatory sequences, that leads to increased constitutive levels; continuous growth stimulation may lead to polyclonal pre-B proliferations, then clonal ...

In this review, we discuss the role that c-MYC plays in the pathogenesis of B-cell lymphomas, the molecular alterations that lead to c-MYC dysregulation, and their effect on prognosis and diagnosis in specific types of B-cell lymphoma.

Apr 7, 2019 · IHC for c-MYC overexpression (>40%) is used to screen and predict c-MYC rearrangements and distinguish diffuse large B-cell lymphoma (DLBCL) NOS from high grade B-cell lymphoma with c-MYC, BCL2 and/or BCL6 rearrangements or so-called “double or triple hit lymphomas” who tend to have aggressive behavior and poor outcome when treated with ...

Immunohistochemistry is a technique that uses antigen-antibody interactions to detect specific proteins in cells. This technique has several essential applications in lymphoma diagnosis, including identifying the cell lineage and phase of ...

Myc overexpression that results from dysregulation in the cell cycle of the Myc protein can be assayed by Western blot or immunohistochemistry (IHC) . There is increasing evidence that Myc overexpression has a prognostic importance that may trump cytogenetic findings.

Thus, FISH is not needed even if more than half of cells are c-MYC positive by PRIME notation. This is the first report describing a correspondence between immunohistochemical findings and chromosomal abnormality, reflecting findings at the protein and gene levels, respectively.

Jan 29, 2016 · Objectives: Immunohistochemistry with anti-MYC antibody (MYC IHC) detects MYC protein in fixed samples of aggressive B-cell lymphomas and, according to the number of positive staining tumor nuclei, facilitates tumor subclassification, predicts underlying MYC rearrangements, and stratifies patient outcome.

Mar 1, 2015 · This review outlines our approach to integrate this new role of C-MYC in a convenient way in routine diagnostics and discusses the application of different techniques such as immunohistochemistry and FISH for C-MYC testing.

Jan 1, 2022 · C-MYC expression by immunohistochemical stain (IHC) is shown to be an independent prognostic factor in DLBCL. In the clinical setting, MYC stain is currently evaluated by manual quantification with a minimum positivity cut-off 40%.

Sep 1, 2015 · Histomorphology, immunohistochemistry (IHC), and genetics are essential tools for the evaluation and classification of lymphoid malignancies. Advances in diagnostic techniques include the development of immunohistochemical assays that …