ATAC-seq identifies accessible DNA regions by probing open chromatin with hyperactive mutant Tn5 Transposase that inserts sequencing adapters into open regions of the genome. [2] [5] While naturally occurring transposases have a low level of activity, ATAC-seq employs the mutated hyperactive transposase. [6]

Oct 26, 2020 · ATAC-seq generates genome-wide regulatory maps that are highly similar to those derived from DNase-seq and MNase-seq 32, while reducing library preparation complexity and hands-on time.

This unit describes Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin.

Feb 3, 2020 · Here, we discuss the major steps in ATAC-seq data analysis, including pre-analysis (quality check and alignment), core analysis (peak calling), and advanced analysis (peak differential analysis and annotation, motif enrichment, …

In ATAC-Seq, genomic DNA is exposed to Tn5, a highly active transposase. Tn5 simultaneously fragments DNA, preferentially inserts into open chromatin sites, and adds sequencing primers (a process known as tagmentation). The sequenced DNA identifies the open chromatin and data analysis can provide insight into gene regulation.

May 15, 2020 · ATAC-seq (assay for transposase-accessible chromatin using sequencing) identifies open chromatin regions all across the genome (1). The method uses the hyperactive transposase Tn5 to cut the accessible chromatin with simultaneous ligation of …

Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) is an effective way to reveal chromatin accessibility at a genome-wide level.

The Assay for Transposase-Accessible Chromatin using high-throughput sequencing (ATAC-seq) is a groundbreaking technique designed to investigate chromatin accessibility and the underlying mechanisms of gene regulation.

Feb 9, 2021 · Many researchers have started using ATAC-Seq as a first-pass screening approach to identify changes in chromatin accessibility between samples, and then they can focus their follow-up experiments based on the results. This article discusses what ATAC-Seq is, it’s history, how it works, and some discoveries enabled by ATAC-Seq. What is ATAC-Seq?

Genome-wide mapping of insertion ends by high-throughput sequencing allows for multidimensional assays of the regulatory landscape of chromatin with a rel-atively simple protocol that can be carried out in hours for a standard sample size of 50,000 cells.