Jun 13, 2011 · Osborn and colleagues report development of a protocol for synthesis and direct insertion of user-defined zinc finger nucleases (ZFN) into versatile expression systems. ZFNs targeting the human α-L-iduronidase (IDUA) gene designed in this manner performed comparably to previously reported ZFNs.

Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. However, construction of target-specific ZFNs is technically challenging. Here, we evaluate a straightforward modular assembly-based approach for ZFN ...

We used Zinc Finger Nucleases (ZFNs) to introduce precise cuts within MLL to examine how a single DNA DSB might lead to chromosomal rearrangements. A ZFN targeting exon 13 within the breakpoint cluster region of MLL was transiently expressed in a human lymphoblast cell line originating from a CML patient.

We have used zinc-finger nucleases to drive the simultaneous genetic modification of both ccr5 and cxcr4 in primary human CD4 + T cells. These gene-modified cells proliferated normally and were resistant to both CCR5- and CXCR4-using HIV-1 in vitro.

To genetically engineer HIV-1 resistance in CD4 + T cells, we assessed whether transient, adenovirus delivered zinc-finger nuclease (ZFN) disruption of genomic cxcr4 or stable lentiviral expression of short hairpin RNAs (shRNAs) targeting CXCR4 mRNAs provides durable resistance to HIV-1 challenge.

Jan 29, 2013 · Treatment of CD4 + T cells with zinc-finger protein nucleases (ZFNs) specifically disrupting chemokine receptor CCR5 coding sequences induces resistance to HIV infection in vitro and in vivo.

May 25, 2008 · In vertebrate cell lines, genomic lesions at a specific site have been introduced using zinc-finger nucleases (ZFNs) 1, 2, 3. Here we adapt this technology to create targeted mutations in the...

Apr 30, 2013 · The ability to use module fingers in the OPEN platform at target sites of 5–7 bp spacer lengths increases the probability of finding a ZFN target site to 1 in 4 bp. These findings significantly expand the range of sites that can be potentially targeted by these custom-engineered proteins.

CompoZr Zinc Finger Nucleases (ZFNs), a class of engineered endonucleases, facilitate targeted genome editing by binding to a user-specified genomic locus and causing a double-strand break (DSB).

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes.