Bimolecular fluorescence complementation
Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions.Author: Anneke Horstman, Isabella Antonia Nougalli Tonaco, Kim Boutilier, Richard G H ImminkPublish Year: 2014www.mdpi.com/1422-0067/15/6/9628Split Green Fluorescent Proteins: Scope, Limitations, and Outlook
Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment …
Versatile protein tagging in cells with split fluorescent protein
Mar 18, 2016 · To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are …
- Author: Daichi Kamiyama, Sayaka Sekine, Benjamin Barsi-Rhyne, Jeffrey Hu, Baohui Chen, Luke A. Gilbert, Hiro...
- Publish Year: 2016
A cautionary note on the use of split-YFP/BiFC in plant ... - PubMed
May 30, 2014 · Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained …
- Author: Anneke Horstman, Isabella Antonia Nougalli Tonaco, Kim Boutilier, Richard G H Immink
- Publish Year: 2014
- Studies of Yfp Split
A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein …
The bimolecular fluorescence complementation (BiFC) assay, also referred to as “split-fluorescent protein” technology (e.g., split-YFP), is one of the most popular and frequently used methods …
Characterization of Split Fluorescent Protein Variants and …
Mar 28, 2018 · Many biotechniques use complementary split-fluorescent protein (sFPs) fragments to visualize protein-protein interactions, image cells by ensemble or single molecule …
Split Fluorescent Proteins for Studying Protein-Protein …
May 3, 2022 · Most frequently, the proteins are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible with multiple FP(11) fragments, even from a …
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Subcellular Localization of Interacting Proteins by Bimolecular ...
Oct 6, 2006 · The BiFC assay for detection of protein-protein interactions in living cells. A molecule of an autofluorescent protein reporter, YFP, is split into two parts, N-terminal (nYFP) …
- Author: Vitaly Citovsky, Lan-Ying Lee, Shachi Vyas, Efrat Glick, Min-Huei Chen, Alexander Vainstein, Yedidya...
- Publish Year: 2006
Bimolecular fluorescence complementation (BiFC) analysis: advances …
The split Citrine at D155 and split Venus at D173 show about 4 - 5 fold and 12 - 15 fold increased BiFC efficiency, respectively, compared to EYFP. Moreover, Citrine and Venus-based BiFC …
Bimolecular Fluorescence Complementation (BiFC) Assay to …
Jul 15, 2023 · In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein–protein interaction in plant cells. Bimolecular fluorescence …
A Cautionary Note on the Use of Split-YFP/BiFC in …
May 30, 2014 · The bimolecular fluorescence complementation (BiFC) assay, also referred to as “split-fluorescent protein” technology (e.g., split-YFP), is one of the most popular and frequently used methods in the plant field to study …
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