
Yellow fluorescent protein - Wikipedia
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission …
How does Pi stacking affect the fluorescence of a protein?
Mar 12, 2021 · I am currenlty studying the GFP varient, YFP and to my understanding, the mutation T203Y produces a face-to-face pi stacking interaction between the aromatic rings of …
A simple and effective method for differentiating GFP and YFP …
Nov 1, 2024 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP has …
1YFP: STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP
Aug 28, 1998 · We have determined the crystal structures of YFP T203Y/S65G/V68L/S72A and YFP H148G to 2.5 and 2.6 A resolution, respectively. Both structures show clear electron …
Structural basis of spectral shifts in the yellow-emission ... - PubMed
Oct 15, 1998 · Results: We have determined the crystal structures of YFP T203Y/S65G/V68L/S72A and YFP H148G to 2.5 and 2.6 A resolution, respectively. Both …
Yellow fluorescent protein Dyes - Probes / BOC Sciences
Yellow fluorescent protein (YFP) dyes are a class of fluorescent proteins that emit yellow fluorescence when excited by light in the blue to green range. These dyes are derived from the …
The Fluorescent Protein Color Palette - Olympus - Life Science
Named after its proficiency in FRET (YFP for e nergy t ransfer), YPet is the brightest yellow fluorescent protein variant yet developed and demonstrates very good photostability. The …
π-Stacking interactions in YFP, quantum mechanics and force …
Aug 17, 2017 · The π-stacking contribution to the absorption red shift observed beween GFP and YFP is quantified. We compare the π -stacking energy landscapes in the S 0 and S 1 states, …
Flow cytometric analysis of CFP–YFP FRET as a marker for in vivo ...
Sep 1, 2005 · CFP–YFP FRET is a stringent and reliable indicator of in vivo protein–protein interactions that allows the steady-state association of functional fusion proteins to be …
CFP eGFP YFP PI Figure 1: The MACSQuant VYB can detect a wide range of fluorescent proteins. Histograms of cells transfected with CFP, eGFP, YFP (top row), non-transfected cells …