XhoI is an isoschizomer of PaeR7I. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol. Impaired by CpG methylation.

Thermo Scientific XhoI restriction enzyme recognizes C^TCGAG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

In molecular biology, XhoI is a type II restriction enzymes that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1. [1] Type II restriction endonucleases are components of prokaryotic DNA restriction-modification mechanisms …

Thermo Scientific FastDigest XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Invitrogen™ Anza™ 8 XhoI is a restriction enzyme that cuts DNA at this recognition site: C^TCGAG, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: PaeR7I, Sfr274I, SlaI.

Unit Definition One unit is defined as the amount of XhoI required to digest 1 μg of lambda DNA-HindIII fragments in 1 hour at 37°C in 50 μL of recommended reaction buffer.

Acronym: Xho Prototype: XhoI Org #: 3070 Organism: Xanthomonas holcicola Organism type: bacteria Organism source: ATCC 13461 (ATCC LINK) Growth Temperature: 26 ° Experimental Evidence: biochemistry Enzyme gene cloned Enzyme gene sequenced Enz Comment: flanking C5 methylation can slow cleavage, ATCTCTCGAGTCTA is cut v. slowly. Molecular Weight ...

Thermo Scientific XhoI restriction enzyme recognizes C^TCGAG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

XhoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the XhoI gene from Xanthomonas holcicola. XhoI activity is impaired by CpG methylation (CG methylase cleaves CT5mCGAG very slowly). The optimum reaction temperature is at 37 °C.

XhoI is an isoschizomer of PaeR7I. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA.