Lancet Infect Dis. 2023 Mar;23 (3):278-280. doi: 10.1016/S1473-3099 (23)00010-5. Epub 2023 Feb 3. 1 Chinese Academy of Sciences Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; Changping Laboratory, Beijing 100871, China.

Feb 29, 2024 · We present receptor binding studies that demonstrate retention of binding contacts with the human ACE2 receptor and a striking decrease in binding to mouse ACE2 due to the revertant R493Q...

May 11, 2023 · In this study, we explored the virological characteristics of XBB, particularly its transmissibility, immune resistance, ACE2 binding affinity, infectivity, fusogenicity, structural information...

May 31, 2023 · Due to the indispensable role of RBD and NTD of spike protein in the regulation of SARS-CoV-2 pathogenesis and immune evasion, we analyzed the binding efficiencies of both wild-type and mutant RBD and NTD with the human ACE2 and mAb, respectively.

Dec 20, 2023 · Here, we show that neutralizing antibody (NAb) evasion drives the convergent evolution of F456L, while the epistatic shift caused by F456L enables the subsequent convergence of L455F through ACE2 binding enhancement and further immune evasion.

In this study, we systematically characterize conformational dynamics, structural stability and binding affinities of the SARS-CoV-2 Spike Omicron complexes with the host receptor ACE2 for BA.2, BA.2.75, XBB.1 and XBB.1.5 variants.

Mar 28, 2025 · AZD3152 derivatives underwent a screening cascade to select for candidates (A) binding to three SARS-CoV-2 RBD variants (Wuhan, BQ.1.1, and XBB.1.5), (B) blocking ACE2-XBB.1 RBD interactions at a level similar to parental AZD3152, and (C) showing improved binding to both XBB.1.5 sentinel mutants D420L and F456L. Pie slices indicate number of ...

Mar 31, 2025 · In comparative infection experiments in A549-ACE2 h cells, XBB.1.16 NSP6 F260L showed 10- and 100-fold lower viral particle production and intracellular viral RNA at 24 and 48 hours post-infection as measured by plaque assay and RT-qPCR, respectively . This confirmed the phenotype of the replicons in fully infectious viral clones.

May 2, 2024 · In this study, we combined AlphaFold-based atomistic structural modeling, microsecond molecular simulations, mutational profiling, and network analysis to characterize binding mechanisms of the SARS-CoV-2 spike protein with the host receptor ACE2 for a series of Omicron XBB variants including XBB.1.5, XBB.1.5+L455F, XBB.1.5+F456L, and XBB.1.5 ...

Apr 7, 2025 · Likewise, infection of Caco-2-ACE2 cells with Omicron subvariants XBB.1.1 and XBB.2.3 was also significantly (p < 0.001) reduced in the presence of camostat mesilate. Together, our results confirm ...