Frizzled and LRP5/6 are Wnt receptors that upon activation lead to stabilization of cytoplasmic β-catenin. In this study, we review the current knowledge of these two families of receptors, including their structures and interactions with Wnt ...

May 6, 2021 · Cripto-1, which is encoded by the Cryptic family 1 gene, is shown to directly bind to LRP5/6, thereby facilitating Wnt3a binding to LRP5/6. Since Cripto-1 is located in lipid rafts, it may also promote caveolin-dependent internalization of LRP5/6, thereby enhancing the canonical Wnt/β-catenin signaling ( Nagaoka et al., 2013 ).

Nov 27, 2017 · We used high-throughput sequencing technology to identify target genes regulated by Wnt3a in osteoblasts and to elucidate the role of Lrp5 and Lrp6 in mediating Wnt3a signaling. Our study identified 782 genes regulated by Wnt3a in primary calvarial osteoblasts.

To understand the molecular basis that accounts for the different signaling activity of LRP5 and LRP6, we generated a series of chimeric receptors via swapping LRP5 and LRP6 cytoplasmic domains, LRP5C and LRP6C, and studied their Wnt signaling activity using biochemical and functional assays.

Apr 22, 2015 · Here we report that Wnt receptor Frizzled (Frz) and theco-receptors LRP5 and LRP6 (LRP5/6) directly interact with each other and this interaction is regulated by the LRP6 ectodomain.

Nov 27, 2017 · We used high-throughput sequencing technology to identify target genes regulated by Wnt3a in osteoblasts and to elucidate the role of Lrp5 and Lrp6 in mediating Wnt3a signaling. Our study identified 782 genes regulated by Wnt3a in primary calvarial osteoblasts.

Wnt3A increased TCF/β-catenin activity in WT and KO5 cells compared to the empty control plasmid, but not in KO6 cells , suggesting that Wnt3A activated canonical Wnt signaling only through LRP6.

Sep 14, 2015 · The Mesd C-terminal region peptide and the full-length Mesd protein block Wnt3a-induced Wnt/β-catenin signaling in LRP5- and LRP6-expressing cells, inhibit Wnt/β-catenin signaling in human breast HS578T cells and prostate cancer PC-3 …

LRP5 and LRP6 are highly homologous and widely co-expressed in embryonic and adult tissues, and they share similar function in mediating Wnt signaling. However, they also exhibit distinct characteristics by interacting with different protein partners.

Dec 21, 2018 · In the present study, the cooperation of SFRP2 and overexpressed FZD5 in transducing WNT3A/β-catenin signaling prompted us to explore the unexpected role of oligomerization between FZD5 and LRP5/6 in ligand-independent β-catenin signaling pathway.