Nov 1, 2012 · TCF/LEFs are multifunctional proteins that use their sequence-specific DNA-binding and context-dependent interactions to specify which genes will be regulated by Wnts. Much of the work to define their actions has focused on their ability to repress target gene expression when Wnt signals are absent and to recruit β-catenin to target genes for ...

T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factors are the major end point mediators of Wnt/Wingless signaling throughout metazoans. TCF/LEFs are multifunctional proteins that use their sequence-specific DNA-binding and ...

The TCF/LEF family (T cell factor/lymphoid enhancer factor family) is a group of genes that encode transcription factors which bind to DNA through a SOX-like high mobility group domain. They are involved in the Wnt signaling pathway , particularly during embryonic [ 2 ] and stem-cell development, [ 3 ] but also had been found to play a role in ...

Jan 3, 2022 · In this article, we comprehensively review the Wnt/β-catenin pathway from the above five aspects in combination with the latest research. Finally, we propose challenges and opportunities for...

Jan 15, 2019 · During canonical Wnt signalling, the activity of nuclear β-catenin is largely mediated by the TCF/LEF family of transcription factors. To challenge this view, we used the CRISPR/Cas9 genome editing approach to generate HEK 293T cell clones lacking all four TCF/LEF genes.

1 day ago · Combo treatment not only repressed the TCF reporter under basal condition but also actively suppressed recombinant WNT3A-induced WNT hyperactivation (Extended Data Fig. 10d). It will be of notable ...

Jan 1, 2014 · Active canonical Wnt signaling results in recruitment of β-catenin to DNA by TCF/LEF family members, leading to transcriptional activation of TCF target genes. However, additional transcription factors have been suggested to recruit β-catenin and tether it to DNA.

Jan 17, 2019 · Transcriptional activation in response to canonical WNT signaling controls processes such as cell fate, proliferation and self renewal of stem cells, as well as contributing to oncogenesis (reviewed in MacDonald et al, 2009; Saito-Diaz et al, 2013; Kim et al, 2013).

May 24, 2011 · The identification of signaling components that are involved in TCF phosphorylation in response to Wnt signals will assist in our understanding of Wnt signaling processes coordinating morphogenesis and cell fate …

Apr 2, 2025 · LEF/TCF are transcription factors that mediate Wnt/β-catenin signaling, regulating gene expression critical for cell fate, proliferation, and differentiation. LEF/TCF acts as a pivotal biomarker to predict Wnt signaling sensitivity [ 251 ], serving as nuclear effectors downstream in the canonical Wnt pathway [ 252 , 253 ].