
Comparative proteome analysis of embryo and endosperm …
Our results revealed that distinct differentially expressed proteins in seed embryo and endosperm are cooperatively involved in seed germination, which provides new insights into the proteomic mechanisms of wheat seed germination.
Wheat seed embryo excision enables the creation of axenic …
Here we investigate wheat seeds as a possible transmission route of endophytes into wheat seedlings and use our findings to develop an axenic system to study the effects of endophyte colonisation on wheat seedlings.
Agrobacterium-mediated direct transformation of wheat mature …
We have efficiently regenerated fertile transgenic wheat plants through organogenesis after Agrobacterium -mediated direct transformation of mechanically isolated mature embryos from field-grown seed.
Comparative proteome analysis of embryo and endosperm …
Apr 8, 2015 · Seed germination is vital to wheat growth and development and directly affects grain yield and quality. In this study, we performed the first comparative proteomic analysis of wheat embryo and endosperm during seed germination.
A multi-omic resource of wheat seed tissues for nutrient ... - Nature
May 10, 2023 · This resource includes a time-series RNA-seq dataset of the embryo, endosperm and outer layers of wheat seeds and their corresponding metabolomic dataset, covering the middle and late stages of...
Comparative metabolome analysis of wheat embryo and …
Jun 1, 2017 · In this study, we performed the first integrated metabolomics analysis of the wheat embryo and endosperm during seed germination using GC-MS. Our results provide novel insights into the biochemical mechanisms of cereal seed germination at the metabolome level.
Wheat seed ageing and embryo protein degradation
Sep 19, 2008 · Wheat seed lots of different viability were obtained by an accelerated ageing technique in which seeds were hydrated up to 12.5–14.5% on a fresh weight basis and stored at 35°C.
Developmental Differences in Embryos of High and Low Protein Wheat ...
Developmental patterns of embryos from high and low protein wheat (Triticum aestivum) grain produced under varied fertilizer conditions were compared. High protein grain produced seedlings 25% heavier with 25% more total RNA, 30% more DNA, 40% more amino acids, 60% more ribosomes, and 80% more soluble protein content than that of low protein seed.
Wheat seed embryo excision enables the creation of axenic
May 6, 2016 · We propose that anatomical restrictions in wheat limit embryo associated vertical transmission, and that bacterial load is carried in the seed coat, crease tissue and endosperm.
Integrated physiology and proteome analysis of embryo and …
Oct 1, 2018 · Metabolic pathway analysis highlighted complex regulatory networks in the embryo and endosperm that regulate wheat seed germination. These results provide new insights into the molecular mechanisms of seed germination. 1. Introduction.